Polyamine Transport by Mammalian Cells and Mitochondria

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Polyamine transport by mammalian cells and mitochondria: role of antizyme and glycosaminoglycans.

The role of antizyme (AZ) and glycosaminoglycans in polyamine uptake by mammalian cells and mitochondria was examined using NIH3T3 and FM3A cells and rat liver mitochondria. AZ is synthesized as two isoforms (29 and 24.5 kDa) due to the existence of two initiation codon AUGs in the AZ mRNA. Most AZ existed as the 24.5-kDa form translatable from the second AUG, but a portion of the 29-kDa AZ fro...

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Introduction The cell concentration of a polyamine depends on both its rates of synthesis and degradation and on its rates of uptake and release. A system for the uptake of putrescine, spermidine and spermine is possessed by many types of cell [ 1 1 , and the uptake of a particular polyamine is often stimulated when its synthesis is inhibited [2]. The transport system is thus involved in the re...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2005

ISSN: 0021-9258

DOI: 10.1074/jbc.m505445200